Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTAIANNHVVSFHYKLTNAEGETLDQSQGEPLAYLHGAGNIIPGLENALTGKTVGDKFTVNVPAAEGYGEYNPDLVQEVPAQMFQGVDNIQPGMQFQAQTDDGVQI-VTVKAVEGDNVIVDANFPLAGQDLTFEVEIVEIREASQEELDHGHVHGAGGHHH 3CGM Chain:A ((3-143)) ---VGQDKVVTIRYTL-QVEGEVLDQGE---LSYLHGHRNLIPGLEEALEGREEGEAFQAHVPAEKAYGPHDPEGVQVVPLSAFPEDAEVVPGAQFYAQDMEGNPMPLTVVAVEGEEVTVDFNHPLAGKDLDFQVEVVKVREATPEEL-------------

General information: TITO was launched using: RESULT: Template: 3CGM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 564 -31539 -55.92 -225.28 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.81 3D Compatibility (PKB) : -55.92 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.691

(partial model without unconserved sides chains): PDB file : Tito_3CGM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3CGM-query.scw PDB file : Tito_Scwrl_3CGM.pdb: