Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAAWTPHVTVATVVEKDGRYLFVEEHSEGFVHTVFNQPAGHVECGETLTEAAIRETLEETGHHIDIDALLGIYTYTPPMFPDRT-YYRFCFLAHVTHVESDPKLDTGIVSAVWMTLDELKESARARSPLVIKAIEDAMKGQHYPLALI--YEHPFSPSLTSHLDA 4NFW Chain:A ((4-149)) -----PHVTVACVVHAEGKFLVVEETING--KALWNQPAGHLEADETLVEAAARELWEETGISAQPQHFIRMHQW---IAPDKTPFLRFLFAIELEQICPTQPHDSDIDCCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFT---------

General information: TITO was launched using: RESULT: Template: 4NFW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 702 -61247 -87.25 -428.30 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -87.25 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.463

(partial model without unconserved sides chains): PDB file : Tito_4NFW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4NFW-query.scw PDB file : Tito_Scwrl_4NFW.pdb: