Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEKNCGSGQSKLKALRMHIYFEVDFQEQAQHYQAVLHSRGVTVDLQPIEKLNARFLRLNPDLALCVDENGLWLSANGMKM-QPDWKAEIPRLKRASLKSEMIARACQLGEKPVLVDATAGLGHDSLLMAYLGAQIQLVERHPILFTLLEDSKAQAQRDPFLSQFMDRIQLIFADS---ASYLKQLDQEEKTVDVVYLDPMFPQRDQNQQAIKKQAQVKKQMQLLHLLLPEDGEMDLGGHLLELAKKVAKRVIVKRPRHAIFLANQEPAHQWQGDACRFDAYFQ 3GDH Chain:A ((48-197)) ---------------------------------------------------------------------------DGIKLDREGWFSVTPE----KIAEHIAGRVSQSFKCDVVVDAFCGVGGNTIQFALTGMRVIAIDIDPVKIAL-------ARNNAEVYGIADKIEFICGDFLLLASFLK--------ADVVFLSPPWGGPD---YATAETFDIRTMMS------PD------GFEIFRLSKKITNNIVYFLPRNA------------------------

General information: TITO was launched using: RESULT: Template: 3GDH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 757 -8688 -11.48 -59.50 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -11.48 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.406

(partial model without unconserved sides chains): PDB file : Tito_3GDH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3GDH-query.scw PDB file : Tito_Scwrl_3GDH.pdb: