Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MWNPNSIIFCFYFIFSILALWGIIEAWVHQSRTETIHPFKAFVHLLAFYLSYLLIPLWFFNLYAGWIGYYSIHETIFIFLLSGILIYARFIEPHMVRVDVHQYRLNPDRHFVKPVKVALIADLHIGLFSGHERQLKIIVKKLN--EQQPDLVVVAGDW--TYEPEDKLVQELSVLKDIKAPVYSVPGNHDE-----QYPGPPIQQLLKDALYYNDVMDIEGKIVEFDEFRLIGIGDLWAGKTDMRSMPDL---PQDKPWLILSHNPDTVDMVPKLPNRPLMLSGHTHGGQVELPLVTNYIMKKVSILGHKRGFYSHEHADVFVTVGTGMVGIPLRFRVPPTIDIIELA 2ZO9 Chain:B ((31-158)) -----------------------------------------------------------------------------------------------------------------------------------------VVSQLNALRERPDAVVVSGDIVNCGRPEEYQVAR-QILGSLNYPLYLIPGNHDDKALFLEYLQPLCPQLGSDANNMRCAVDDFATRLLFIDSSRAGTSKGWLTDETISWLEAQLFEGGDKPATIFMHHP----------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2ZO9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 439 12893 29.37 111.15 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain B : 0.51 3D Compatibility (PKB) : 29.37 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.51 QMean score : 0.362

(partial model without unconserved sides chains): PDB file : Tito_2ZO9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ZO9-query.scw PDB file : Tito_Scwrl_2ZO9.pdb: