Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKERFQMKKYQWGIVGLGTIAHEFA---ESFNQETSELTAVASRTSEKAENFAHRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKKELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDPKNRFFNPELAGGALLDIGTYAVSFARFFLSSQ-PEVVASTMVPFETGVDEQSVTILRNKENEL-AAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFI-------ESGNTSEAMNYEIKNMVHMIEGDL---PNRSLFLTKDVIEILDQMQVLWKNM 3OHS Chain:X ((4-322)) ----------RWGIVSVGLISSDFTAVLQTLPRSEHQVVAVAARDLSRAKEFAQKHDIPKAYGSYEELAKDPNVEVAYVGTQHPQHKAAVMLCLAAGKAVLCEKPMGVNAAEVREMVTEARSRGLFLMEAIWTRFFPASEALRSVLAQGTLGDLRVARAEFGKNLTHVP--RAVDWAQAGGALLDLGIYCVQFISMVFGGQKPEKISVMGRRHETGVDD-TVTVLLQYPGEVHGSFTCSITAQLSNTASVSGTKGMAQLLN-PCWCPTELVVKGEHKEFLLPPVPKNCNFDNGAGMSYEAKHVRECLRKGLKESPVIPLVESELLADILEEVR------

General information: TITO was launched using: RESULT: Template: 3OHS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain X - contact count / total energy / energy per contact / energy per residue : 1721 -7030 -4.08 -23.12 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain X : 0.78 3D Compatibility (PKB) : -4.08 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.540

(partial model without unconserved sides chains): PDB file : Tito_3OHS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3OHS-query.scw PDB file : Tito_Scwrl_3OHS.pdb: