Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAAGGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSKEEAEELKAKLEEVGASVTVK 1CTF Chain:A ((7-74)) ------------------------------------------------------EFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKDDAEALKKALEEAGAEVEVK

General information: TITO was launched using: RESULT: Template: 1CTF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 281 -34613 -123.18 -509.01 target 2D structure prediction score : 0.87 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -123.18 2D Compatibility (Sec. Struct. Predict.) : 0.87 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.749

(partial model without unconserved sides chains): PDB file : Tito_1CTF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1CTF-query.scw PDB file : Tito_Scwrl_1CTF.pdb: