Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRIEMMPEVDGMVPNRWLSTLTI----NDYNSIDQVVNV--------------INKLNEKNIETRALWKPLHLQPVFQGVKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK 4ZTC Chain:A ((2-372)) ----------------RFFLSPPHMGGNELKYIEEVFKSNYIAPLGEFVNRFEQSVKDYSKSEN-ALALNSATAALHLALRVAGVKQDDIVLASSFTFIASVAPICYLKAKPVFIDCD-ETYNIDVDLLKLAIKECEKK---PKALILTHLYGNAAKMDEIVEICKENDIVLIEDAAEALGSFYKNKALGTFGEFGVYSYNGNAIITTSGGGMLIGKNKEKIEKARFYSTQARENCLHYEHLDYGYNYRLSNVLGAIGVAQMEVLEQRVLKKREIYEWYKE-FLG-EYFSFLDELENSRSNRWLSTALINFDKNELNSCQKDINISQKNITLHPKISKLIEDLKNKQIETRPLWKTMHAQEVFKGAKAYLNGN----SELFFQKGICLPSGTAMSKDD-------------

General information: TITO was launched using: RESULT: Template: 4ZTC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2112 -129377 -61.26 -366.51 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.84 3D Compatibility (PKB) : -61.26 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.547

(partial model without unconserved sides chains): PDB file : Tito_4ZTC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZTC-query.scw PDB file : Tito_Scwrl_4ZTC.pdb: