Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPEVLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQRELTSNEDNLFLNIHDKFLALLLTDDELITNLLEVE 5HZ1 Chain:B ((461-538)) --SLLGTTLPKSLKFIDFSDNALSSTLPPGIGLLTE-LTKLNLAKNRLSGEIP----REISTCRSL---QLLNLGENDFSGEIPDELG-----------------------------------------------

General information: TITO was launched using: RESULT: Template: 5HZ1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 304 -21968 -72.26 -281.64 target 2D structure prediction score : 0.40 Monomeric hydrophicity matching model chain B : 0.66 3D Compatibility (PKB) : -72.26 2D Compatibility (Sec. Struct. Predict.) : 0.40 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.093

(partial model without unconserved sides chains): PDB file : Tito_5HZ1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5HZ1-query.scw PDB file : Tito_Scwrl_5HZ1.pdb: