Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEERFDWMTFGDDVSGAQAGSAAPPSTGADEDVPIFLREDVGMVRAGASHATAASESTGVDSENGAPAFLAVCPGADVAPRLSGGWSSEGSPVWDGSLRQQQKPSSTAALGHPGGAGDVQAAPSAALYADQPSAAVLHFTGDSGRSWGALHRVRILGKGSYG-CATLYSMKDAASGGVEPTHAVVVKDINMQTMLNPAEEVLAVQNELKVLRTVLGHPNLVQYVDALFDTRPPTYPMSFIMMEYCAGGDLAAVMDGRRSDSATTNGAPSSPPSIPLAITTASTAAGPDILTEPCVASLFIQVAVALQSLHTE-YGILHRDVKPHNIFLLEDGI-TVRLGDFGISTQLDRVGDTAKEACGSPYYMAPELFEERAYGAAADVWSLGVVFYQLIARQLPFTAASAAELRSLVCRGRCTPLHDLQNEAASRYSRQFKELVSSLLTVDAAARPTLRRVLRHKYVRESLKYVPASVLTAKKPLPPARVPSAGAMPSSSLQRFSADGLYAGLFGSDVVEQAVSQAVHAGVYPIVSRVHSA 2XNP Chain:A ((14-273)) -----------------------------------------------------------------------------------------------------------------------------------------------------------IGTG-YGRCQKIRRKSDGK--------ILVWKELDYGSMTEAEKQMLV--SEVNLLRE-LKHPNIVRYYDRIIDRTNTTL---YIVMEYCEGGDLASVI---------TKGTKER-----------------QYLDEEFVLRVMTQLTLALKECHRRS----HRDLKPANVFL--DGKQNVKLGDFGL-------------FVGTPYYMSPEQMNR---NEKSDIWSLGCLLYELCALMPPFTAFSQKELAGKIREGKFRRI-------PYRYSDELNEIITRMLNLKDYHRPSVEEILENPLILE------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2XNP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1016 -53868 -53.02 -231.19 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -53.02 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.520

(partial model without unconserved sides chains): PDB file : Tito_2XNP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2XNP-query.scw PDB file : Tito_Scwrl_2XNP.pdb: