Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence SDPYFSPPSESQKCFYTIYRVESINGEKVLVREGVDHSYTGW-------DAEIKKINVHFDKECNPQYQSSQWVFFKGVRKERHIYPDR 1J5O Chain:A ((113-184)) -DAYFSVPLDEDFRKYTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQSSMTKILEPFKKQ-NPDIVIYQYI---------------

General information: TITO was launched using: RESULT: Template: 1J5O.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 133 -4000 -30.08 -61.54 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -30.08 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.227

(partial model without unconserved sides chains): PDB file : Tito_1J5O.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1J5O-query.scw PDB file : Tito_Scwrl_1J5O.pdb: