Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAFRQALQLAACGLAGGSAAVLFSAVAVGKPRAGGDAEPRPAEPPAWAGGARPGPGVWDPNWDRREPLSLINVRKRNVESGEEELASKLDHYKAKATRHIFLIRHSQY-HVDG--------SL-EKDRTLTPLGREQAELTGLRLASLGLKFNKIVHSSMTRAIETTDIISRHLPGVCKVSTDLLRE--GAPIEP---DPP-------------VSHWK------PEAVQYYEDGARIEAAFRNYIHRADARQEEDSYEIFICHANVIRYIVCRALQFPPEGW------LRLSLNNGSITHLVIRPN-----------GRVALRTLGDTGFMPPDKITRS 2YN0 Chain:A ((2-250)) ------------------------------------------------------------------------------------------------VNTIYIARHGYRSNWLPEGPYPDPLTGIDSDVPLAEHGVQQAKELAHYLLSLDNQPEAAFASPFYRCLETVQPIAKLLE-IPVYLERGIGEWYRPDRKPVIPVPAGYEILSKFFPGVISQEWDSTLTPNEKGETEQEMYMRFKKFWPLFIERVEKEYPNVECILLVTHAASKIALGMSLLGYDNPRMSLNENGDKIRSGSCSLDKYEILKKSYDFTYIPFSDRKWVLTMNGNTEFLSSGE----

General information: TITO was launched using: RESULT: Template: 2YN0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 951 14039 14.76 74.68 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : 14.76 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.519

(partial model without unconserved sides chains): PDB file : Tito_2YN0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2YN0-query.scw PDB file : Tito_Scwrl_2YN0.pdb: