Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence WNDG---GFVNKQQAHDLLINKPDGTFLLRFSDSEIGGITIAW----KFDSPERNLWNLKPFTTRDFSIRSLADRLGDLSYLIYVF 4Y5W Chain:A ((424-503)) WSDRLIIGFISKQYVTSLLLNEPDGTFLLRFSDSEIGGITIAHVIRGQDGSPQ--IENIQPFSAKDLSIRSLGDRIRDLAQL----

General information: TITO was launched using: RESULT: Template: 4Y5W.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 233 12547 53.85 171.87 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : 53.85 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.616

(partial model without unconserved sides chains): PDB file : Tito_4Y5W.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4Y5W-query.scw PDB file : Tito_Scwrl_4Y5W.pdb: