Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence WYFGKITRRESERLLLNAENPRGTFLVRESETTKGAYCLSVSDFDNAKGLNVKHYKIRKLDSGGFYITSRTQFNSLQQLVAYY 1KSW Chain:A ((67-149)) WYFGKITRRESERLLLNAENPRGTFLVRESETTKGAYCLSVSDFDNAKGLNVKHYKIRKLDSGGFYITSRTQFNSLQQLVAYY

General information: TITO was launched using: RESULT: Template: 1KSW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 298 -43197 -144.96 -520.45 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 1.00 3D Compatibility (PKB) : -144.96 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 1.00 QMean score : 0.646

(partial model without unconserved sides chains): PDB file : Tito_1KSW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1KSW-query.scw PDB file : Tito_Scwrl_1KSW.pdb: