Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIHFSINKNLFLQALNITKRAISSKNAIPILSTVKIDVTNEGVTLIGSNGQISIENFISQKNEDAGLLITSLGSILLEASFFINVVSSLPDVTLDFKEIEQNQIVLTSGKSEITLKGKDSEQYPRIQEISASTPLILETKLLKKIINETAFAASTQESRPILTGVHFVLSQHKELKTVATDSHRLSQKKLTLEKNSDDFD--VVIPSRSLREFSAVFTDDIETVEIFFANNQ-------ILFRSENISFYTRLLEGNYPDTDRLIPTDFNTTITFNVVNLRQSMERARLLSSATQNGTVKLEIKDGVVSAHVHSPEVGKVNEEIDTDQVTGEDLTISFNPTYLIDSLKALNSEKVTISFISAVRPFTLVPADTDE-----------DFMQLITPVRTN 5AH2 Chain:A ((15-398)) -LKFRVVREDFADAVAWVARSLPTRPTIPVLAGVLLTGTDEGLTISGFDYEVSAEVKVSAE-------IASAGSVLVSGRLLSDITKALPAKPVEVS-VEGTRVSLTCGSARFSLPTLAVEDYPALPALPEETGVI-ASDLFAEAIGQVAVAAGR---LPMLTGIRVEIS-GESVVLAATDRFRLAVRELTWVTTAGDVEAAVLVPAKTLAEAAKAGTDGNQ-VHLALGSGASVGKDGLLGIRSEGKRSTTRLLDAEFPKFRQLLPAEHTAVATIGVAELTEAIKRVALV--ADRGAQIRMEFSDDTLKLSAGADDVGRAEEDLPVD-FAGEPLTIAFNPTYLTDGLGSLHSERVTFGFTTPSRPAVLRPAGE--GGSGPFPAAKTDYVYLLMPVR--

General information: TITO was launched using: RESULT: Template: 5AH2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1872 -111320 -59.47 -312.70 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : -59.47 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.538

(partial model without unconserved sides chains): PDB file : Tito_5AH2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5AH2-query.scw PDB file : Tito_Scwrl_5AH2.pdb: