Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLLSIAISSYNAAAYLHYCVES-LVIGGEQVGILIINDGSQDQTQEIAECLASKYPNIVRAIYQENKCHGGAVNRGLAEASGRYFKVVDSDDWVDPRAYLKILETLQELESKGQEVDVFVTNFVYEKEGQSRKKSMSYDSVLPVRQIFGWDQVGNFSKGQYTMMHSLIYRTDLLRASQF 2Z86 Chain:A ((374-504)) VPLVSIYIPAYNCSKYIVRCVESALNQTITDLEVCICDDGSTDDTLRILQEHYANHPR-VRFISQKNKGIGSASNTAVRLCRGFYIGQLDSDDFLEPDA---VELCLDEFRKDLSLACVYTTNRNIDREGN-------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2Z86.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 585 -28510 -48.74 -226.27 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -48.74 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.395

(partial model without unconserved sides chains): PDB file : Tito_2Z86.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2Z86-query.scw PDB file : Tito_Scwrl_2Z86.pdb: