Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKQSLFFVPGIILIGVSLRTPFTVLPIILGNISQGLEVEVSSLGVLTSLPLLMFTLFSPFSTQLAQKIGLEHLFTYSLFFLTIGSLIRL-I---N-LPLLYLGTLMVGASVAVINVLLPSLIQANQP-KKIGFLTTLYVTSMGIATALASYLAVPITQA--------------------------SSWKGLILLLTLLCLATFLVWLPNH-RYNHRLA-PQ----TKQKSQIKVMRNKQVWAIIIFSGFQSLIFYTVMTWLPTMSIHA--GLSSHEAGLLTSILSLISIPFSMTIPSLTTSLSTRNRQLMLTLVSLAGVIGISMLFFPINNFIYWLAIHLLIGTATSALFPYLMVNFSLKTSAPEKTAQLSGLSQTGGYILAAFGPTLFGYSFDLFHSWVPSVAALLLIDILMTVALFTVDRADKIL 3O7Q Chain:A ((42-435)) ----------------------NNLNDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYALGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQTFNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAIVLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTACWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFA-PHK-VLAAYA-LIAMALCLISAFAGGH-VGL-IALTLCSAFMSIQYPTIFSLGIKNLGQDTKYG--SSFI-VMTIIGGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQT----

General information: TITO was launched using: RESULT: Template: 3O7Q.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1558 -206850 -132.77 -584.32 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -132.77 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.306

(partial model without unconserved sides chains): PDB file : Tito_3O7Q.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3O7Q-query.scw PDB file : Tito_Scwrl_3O7Q.pdb: