Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVMSDNTKYIFLSPHLDDAIFSCGDYISKLTSEGEIVLVITIFSGYPLSQQLQPSAKQFHKLCNLGKYPIEE----RKKEDRLACERLQCDFRHLSYYECLYRKDRNGNFLYRHI----------YSELKNEDTLKN--DIIKELLMHLDDKCVVYCPLSLGDHVDHVFVNSIGR-ALEFMRYKVIYYEDFPY-VSDSSMVSY-----MGKTKELKMYQEELDEKHYIDRISSILCYKSQILIIWKSVEKLLNNIKELYLRNG--AAYSIRFWIKK 3DFM Chain:A ((8-265)) ------TRLLAISPHLDDAVLSFGAGLAQAAQDGANVLVYTVFAGA-AQPPYSPAAQRMHTIWGLA--PDDDAVLYRRKEDIAALDHLRVAHRHGRFLDSIYRKLPDGRWLTAHVEGRQKLAVNDHSPDSDHDLVGEVADDIRSIIDEFDPTLVVTC-AAIGEHPDHEATRDAALFATHEKNVPVRLWEDLPYAVFKSGAVELPQGFRLGSADVSSVKPEMRSQK-----FQAVERYSSQMVLLNGSENNLFDRLDEHARQNAPHGGYGETTW---

General information: TITO was launched using: RESULT: Template: 3DFM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1163 -34809 -29.93 -149.39 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -29.93 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.426

(partial model without unconserved sides chains): PDB file : Tito_3DFM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3DFM-query.scw PDB file : Tito_Scwrl_3DFM.pdb: