Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKIISHRYFIIVFLLVIADQKFSVLVLRSDLVAGLSDFAYYLSDMMLNFLVVLFALIAMIWSGKWQKINSRKFKGSYLFYSFLALLAFVAWNFVTFFLFPPTRNEISYQHAAPTFTGATAFLMYFFYPVIAGPIFEDMIYRGLVMTALEKGKKWGLDVLGSAVLFGVSHISNHG---------------WVLTDFVFYMGGGLIFAVLFRMT-KSIYWPIGLHIVYNGIGQLLMLL 4CAD Chain:F ((127-237)) ---------------------------------------------------------------------------------------------------------------------------VPVLFLLLLAATFEELGWRGYAFDSLQSRYSLFKASILFGIFWSLWHFPLIFVNNSYQYEIFNQSIWYGLNFFLSILPMGIIITWMCLKNRKSIILAIIFHFLINLNQELL---

General information: TITO was launched using: RESULT: Template: 4CAD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 215 -34428 -160.13 -362.40 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain F : 0.52 3D Compatibility (PKB) : -160.13 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.52 QMean score : 0.058

(partial model without unconserved sides chains): PDB file : Tito_4CAD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4CAD-query.scw PDB file : Tito_Scwrl_4CAD.pdb: