Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMKVFLQNRDFRQLTINQWISTLGDTIFYLAFLNYVADASF-APLAILLITISETLPQ-VLQIFLGVLADFQHHRVLKYTVISFAKFLLYSIVSLSLSG-------QSFSLLLVAFICLINLLSDTLSYFSG-----AMLTPIFIRIIGQDHLAEAIGFKQSTVSLVKTISNILGGVLLGILSIQF---ISLLNALTFLIAFLGILFI-KTDLLKVEKTINYQEGLSVKSFCQHLLQSSKLIWNMNKVLLVLFIISTSQAVINVTVPVSTLFLRNQPFLNLQTGQSLALLSTFELSALIVGSLVSGYLQHTISIKTALYASLVIQLL------LLVGF-ATVRFDWILIFSTLDAFFAGVLSPRLQELVFKQIPEESMGAVQSSISAITVVLPSLFTISLVTIATSFGTLAVSFVLLLFLLVAFVMLLNIRESI 5AYO Chain:A ((4-419)) ----QSLLRIETQLLLGRLLTRSGDQAWDFVVPFALLVIFPGKLQVAAFYYLIVKIGTFLLTPSSGKWIDTH-PRIQVVKWGVWLQFFAILAGMVFFGMLDGLVRAGGRESWLLSVLFIALALSGVMASLGSQITDISVGNDLAPSLVAPEKLTHFNSWLRRIDLATEVGAPILAGALFAFHPEQLPLAGLFLIGLWNLVSFVPEYFLLRNVIQRSGLKIK--VLT-----------------SDPIFWLILSYALLWLSVLSPHGVLLAAYL-KDE-MRLP-ETEIGLFRGLGAVFGLISTVSFPYLVRRLGLISSSRWHLGFQGVTLGIAVTAFAMGSTASVYVFLGCILLSRVGLYGFSNGEFELRQRLIPEGRRGELNSLSSLTTTSATLILFSAGSLLPQTEDFKYLVYVSLAAVLLANVV--------

General information: TITO was launched using: RESULT: Template: 5AYO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1524 -208049 -136.52 -556.28 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -136.52 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.280

(partial model without unconserved sides chains): PDB file : Tito_5AYO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5AYO-query.scw PDB file : Tito_Scwrl_5AYO.pdb: