Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAEKMEKTGQILQMQLKRFSRVEKAFYFSIAVTTLIVAISIIFMQTKLLQVQNDLTKINAQIEEKKTELDDAKQEVNELLR------AERLKEIANSHDLQLNNENIRIAE 4R6G Chain:A ((287-348)) ---------------------------------------------TTLNLSNNQLTSLPKGVLERLTNLKTLNLSNNQITKEVCRHVAELLKQAASLHELHLSNNNI----

General information: TITO was launched using: RESULT: Template: 4R6G.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 173 8336 48.18 148.86 target 2D structure prediction score : 0.20 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : 48.18 2D Compatibility (Sec. Struct. Predict.) : 0.20 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.173

(partial model without unconserved sides chains): PDB file : Tito_4R6G.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4R6G-query.scw PDB file : Tito_Scwrl_4R6G.pdb: