Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKKVGVGQAHSKIILIGEHAVVYGYPAI----------------SLPLLEVEVTCKVVPAESPWRLYEEDTLSMAVYASLEYLNITEACIRCEIDSAIPEKRGMGSSAAISIAAIRAVFDYYQADLPHDVLEILVNRAEMIAHMNPSGLDAKTCLSDQPIRFIKNVG---FTELEMDLSAYLVIADTGVYGHTREAIQVVQN-KGKDALPFLHALGELTQ---QAEIAISQKDAEGLGQILSQAHLHLKEIGVSSLEADSLVETALSHGALGAKMSGGGLGGCIIALVTNLTHAQELAERLEEKGAVQTWIESL 2HFU Chain:A ((10-312)) -SKTTGKNIGYGKVILFGEHFVVHGAEAIVAGISEYTECRLEINPGVPGLQVDDQRPAIPGYIAQKRDEQIKAHQLVLDHLK-VDLSGDGLKMFIGGPLVPSSGIGASASDVVAFSRALSELYQLNLTDEEVNLSAFVGEGGYHGTPSGADNTAATYGGLILYRRQNGKSVFKPIAFQQRLYLVVVGTGINASTAKVVNDVHKMKQQQPVQFKRLYDNYTHIVSQAREALQKGDLQRLGQLMNANHDLCRQIDVSCRELESIVQTCRTYGALGAKLSGTGRGGIAVALAASSDQRDAIVKGLKAK----------

General information: TITO was launched using: RESULT: Template: 2HFU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1501 22929 15.28 81.89 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : 15.28 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.498

(partial model without unconserved sides chains): PDB file : Tito_2HFU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2HFU-query.scw PDB file : Tito_Scwrl_2HFU.pdb: