Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKADTIFKENIERILKEGVFSEQ--ARPKY-KDGTVANSKYVTGAFSEYDLSKGEFPITTLRPIAIKSAIKEVLWIYQDQSNSLEVLNDKYNVHYWNDWEVGDTGTIGERYGAVVKKH---------DIINKLLKQLETNPWNRRNIISLWDYQAFEETDGLLPCAFQTMFDVRRVDGEIYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWKVGKFFYFINNLHIYDNQFEQAQELLRREPSNCQPRLVLNVPDGTNFFDIKAEDFELVDYDPVKPQLKFDLAI 1BSF Chain:A ((2-279)) -TQFDKQYNSIIKDIINNGISDEEFDVRTKWDSDGTPAHTLSVISKQMRFDNS--EVPILTTKKVAWKTAIKELLWIWQLKSNDVNDLN-MMGVHIWDQWKQED-GTIGHAYGFQLGKKNRSLNGEKVDQVDYLLHQLKNNPSSRRHITMLWNPDELDAM-ALTPCVYETQWYVKH--GKLHLE--VRARSNDMALGNPFNVFQYNVLQRMIAQVTGYELGEYIFNIGDCHVYTRHIDNLKIQMEREQFEA-PELWIN-PEVKDFYDFTIDDFKLINYKH-GDKLLFEVAV

General information: TITO was launched using: RESULT: Template: 1BSF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1270 5935 4.67 22.31 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : 4.67 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.553

(partial model without unconserved sides chains): PDB file : Tito_1BSF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1BSF-query.scw PDB file : Tito_Scwrl_1BSF.pdb: