Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MISNKVDITLANFTVTDERKKQVDFALPYMKVSLGVVSPKTG-LITDVKQLEGKTLIVTKGTTAETYFEKNHPE--IKLQKYDQYSDSYQALLDGRGDAFSTDNTEVLAWALE--NKGFE-VGITSLGDPDTIAAAVQKGNQELLDFINKDIEKLGKENFFHKAYEKTLHPTYGDAAKADDLVVEGGH 4ZV2 Chain:A ((67-229)) --SGKIDVVIAGMTITEERKKQVDFSDPYFEAGQAIVVKKGNDSIKSLEDLKGKKVGVQLGSTSEQHVKKVA--AGVKVKKFDNFSEAFQELKSGRVDAVVTDNAVALAYVKQNPNAGVKIVGETFSGEPYGI--AVRKGNSELLEKINKALEEMKKDGTYDKIYEK---------------------

General information: TITO was launched using: RESULT: Template: 4ZV2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 686 51718 75.39 333.66 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : 75.39 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.555

(partial model without unconserved sides chains): PDB file : Tito_4ZV2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZV2-query.scw PDB file : Tito_Scwrl_4ZV2.pdb: