Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MWYNRAMKHFDTIVIGGGPAGMMATISSSFYGQKTLLIEKNRKLGKKLAGTGGGRCNVTNNGSLDNLLAG-IPGNGRFLYSVFSQFDNHDIINFFTENGVKLKVEDHGRVFPASDKSRTIIEALEKKITELGGQVATQIEIVSVKKVDD----QFVLKSADQTFTCEKLIVTTGGKSYPSTGSTGFGHEIARHFKHTITDLEAAESPLL---TDFPHKALQGISLDDVTLSYGKHVITHDLLFTHFGLSGPAALRMSSFVKGGEVLSLDVLPQLS-EKDLVTFLEENREKSLKNALKTLLPERLAEFFV-QGY--PEKVKQLTEKEREQLVQSIKELKIPVTGKMSLAKSFVTKGGVSLKEINPKTLESKLVPGLHFAGEVMDINAHTGGFNITSALCTGWVAGSLHYD 2GQF Chain:A ((3-395)) -------QYSENIIIGAGAAGLFCAAQLAKLGKSVTVFDNGKKIGRKILMSGGGFCNFTN---LEVTPAHYLSQNPHFVKSALARYTNWDFISLVAEQGITYHEKELGQLF-CDEGAEQIVEMLKSECDKYGAKILLRSEVSQVERIQNDEKVRFVLQVNSTQWQCKNLIVATGGLSMPGLGATPFGYQIAEQFGIPVIPPRASLVPFTYRETDKFLTALSGISLPVTITALCGKSFYNQLLFTHRGISGPAVLQISNYWQPTESVEIDLLPNHNVEEEINQAKQSSPKQMLKTILVRLLPKKLVELWIEQGIVQDEVIANISKVRVKNLVDFIHHWEFTPNGTEGYRTAEVTMGGVDTKVISSKTMESNQVSGLYFIGEVLDVTGWLGGYNFQWAWSSAYACA-----

General information: TITO was launched using: RESULT: Template: 2GQF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2108 6788 3.22 17.82 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : 3.22 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.484

(partial model without unconserved sides chains): PDB file : Tito_2GQF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2GQF-query.scw PDB file : Tito_Scwrl_2GQF.pdb: