Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MALLAEFYSKEGNMNTNLASFIVGLIIDENDRFYFVQK-DGQTYALAKEEGQ-------HTVGDTVKGFAYT--DMKQKLRLTTLEV-----------TATQDQFGWGRVTEVRKDLGVFVDTGLPDKEIVVSLDILPE--LKELWPKKGDQLYIRL-EVDK-KDRIWGLLAYQEDF---QRLARPAYNNMQNQNWPAIVYRLKLSGTFVYLPENNMLGFIHPSERYAEPRLGQVLDARVIGFREVDRTLNLSLKPRSFEMLENDAQMILTYLESNGGFMTLNDKSSPDDIKATFGISKGQFKKALGGLMKAGKIKQDQFGTELI 4Q7J Chain:D ((16-179)) -------------IETRPGSIVRGVVVAIDKDVVLVDAGLKSESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAYEDAETVTGVINGKV-KGGFTVELN--GIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLDQKRNNVVVSRRAVIESENS-------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4Q7J.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 332 -4278 -12.89 -31.46 target 2D structure prediction score : 0.45 Monomeric hydrophicity matching model chain D : 0.60 3D Compatibility (PKB) : -12.89 2D Compatibility (Sec. Struct. Predict.) : 0.45 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.367

(partial model without unconserved sides chains): PDB file : Tito_4Q7J.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4Q7J-query.scw PDB file : Tito_Scwrl_4Q7J.pdb: