Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIRLETMKVVAQEEIAPAIFELVLEGEMVEAMR-AGQFLHLRVPDDAHLLRRPISISSIDKANKQCHLIYRI--EGAGTAIFSTLSQGDTLDVMGPQGNGFDLSDLDEQNQVLLVGGGIGVPPLLEVAKELHERGVKVVTVLGFANKDAVILKTELAQYGQVF--VTTDDGSYGIKGNVSVVINDLDSQFDAVYSCGAPGMMKYINQTFDDHPRAYLSLESRMACGMGACYACVLKVPESETVSQRVCEDGPVFRTGTVVL 1EP3 Chain:B ((6-255)) ----EMMTVVSQREVAYNIFEMVLKGTLVDEMDLPGQFLHLAVPNGAMLLRRPISISSWDKRAKTCTILYRIGDETTGTYKLSKLESGAKVDVMGPLGNGFPVAEVTSTDKILIIGGGIGVPPLYELAKQLEKTGCQMTILLGFASENVKILENEFSNLKNVTLKIATDDGSYGTKGHVGMLMNEIDFEVDALYTCGAPAMLKAVAKKYDQLERLYISMESRMACGIGACYACVEHDKEDESHALKVCEDGPVF-------

General information: TITO was launched using: RESULT: Template: 1EP3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1364 -33766 -24.76 -137.82 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain B : 0.85 3D Compatibility (PKB) : -24.76 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.502

(partial model without unconserved sides chains): PDB file : Tito_1EP3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1EP3-query.scw PDB file : Tito_Scwrl_1EP3.pdb: