Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKNKRIFKDFQASKMSLNIYTSPLLAFVFVFIGEFVAFTLYGIGLLALIGLARNFGEAGQNLATYLQTLHQSLTDKTSDFRLILGLLAFGFILNTVFRWTRKVEKRPIRTLGFYRENFLSNLLKGFSLGLALFLLTLLGLVVLGQYRLESIHLNPYSLAFVVFTIPFWILQGTAEEVVARAWLLPQLASRTNLKLAILISSLFFTLLHMGNSGLTPLSLVNLFLFGVAMALYLLKTDTVWGVAGIHGAWNFAQGNLFGILVSGQPSGTSLMTFLPQGNQDWLSGGSFGIEGSIMTSLVLLLLIVYLANKLKKENERM 5FN5 Chain:C ((72-99)) -------------------------------------------------------------------------------------------------------------------------------------------------------------------LIFGAAVSVLLQEV-FRFAYYKLLKKADE-----------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5FN5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 5 -434 -86.80 -15.50 target 2D structure prediction score : 0.82 Monomeric hydrophicity matching model chain C : 0.40 3D Compatibility (PKB) : -86.80 2D Compatibility (Sec. Struct. Predict.) : 0.82 1D Compatibility (Hydrophobicity) : 0.40 QMean score : 0.263

(partial model without unconserved sides chains): PDB file : Tito_5FN5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5FN5-query.scw PDB file : Tito_Scwrl_5FN5.pdb: