Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRKRDRHQLIKKMITEEKLSTQKEIQDRLEAHNVCVTQTTLSRDLREIGLTKVKKND---MVYYVLVNETEKIDLVEFLSHHLEGVAR--AEFTL---------VLHTKLGEASVLANIVDVNKDEWILGTVAGANTLLVICRDQHVAKLMEDRLLDLMKDK 3ERE Chain:D ((18-156)) --RAGRQARIVAILSSAQVRSQNELAALLAAEGIEVTQATLSRDLEELGAVKLRGADGGTGIYVV----PEDGSPVRGVSGGTDRMARLLGELLVSTDDSGNLAVLRTPPGAAHYLASAIDRAALPQVVGTIAGDDTILVVAREP-----------------

General information: TITO was launched using: RESULT: Template: 3ERE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 458 -5762 -12.58 -46.09 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain D : 0.74 3D Compatibility (PKB) : -12.58 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.471

(partial model without unconserved sides chains): PDB file : Tito_3ERE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ERE-query.scw PDB file : Tito_Scwrl_3ERE.pdb: