Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKLQFIASCPQHGVLDHPPNECHITQEYVAALLFKQLGNYGFDA--QHIVHEHE----KVEVSIDNHLL-PLSITCQQTDHDGHLMCEISANPDEEQDWFEKIETQSIIRQLAQAVENSLKADHSFSAFEWKS 2E3M Chain:A ((371-459)) -------------------------VEEMVQNHMTYSLQDVGGDANWQLVVEEGEMKVYRREVEENGIVLDPLKATHAVKGVTGHEVCNYFWNVDVRNDWETTIENFHVVETLA--------------------

General information: TITO was launched using: RESULT: Template: 2E3M.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 142 8633 60.79 105.27 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 60.79 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.119

(partial model without unconserved sides chains): PDB file : Tito_2E3M.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2E3M-query.scw PDB file : Tito_Scwrl_2E3M.pdb: