Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLFSGSSMSEQIFIQGPVGKIELFVDRPEGEIKGFAVVCHPHPLQGGTPQHKVPALLTQIFNEYGCIVYRPSFRGLGGSEGVHDEG--HGETEDILAVIEHVRKLHAGLPFYAGGFSFG--SHVLAKCHAQ-----LSPELQPVQLILCGLPTATVVGLRH-----------YKTPEIQGD--------------------ILLIHGEQDDITLLSDAIEWAKPQKH-PITILSGANHFFTGYLKQLRQIITRFIIMK 2WTM Chain:A ((12-246)) -------------------KLNAYLDMPKNNPEKCPLCIIIHGFTGHSEERHIVAV-QETLNEIGVATLRADMYGHGKSDGKFEDHTLFKWLTNILAVVDYAKKLDFVTDIYMAGHSQGGLSVMLAAAMERDIIKALIP-LSPAAMIPEIARTGELLGLKFDPENIPDELDAWDGRKLKGNYVRVAQTIRVEDFVDKYTKPVLIVHGDQDEAVPYEASVAFSKQYKNCKLVTIPGDTHCYDHHLELVTEAVKEFML--

General information: TITO was launched using: RESULT: Template: 2WTM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1038 -30472 -29.36 -157.07 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -29.36 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.333

(partial model without unconserved sides chains): PDB file : Tito_2WTM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2WTM-query.scw PDB file : Tito_Scwrl_2WTM.pdb: