Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQDTPLIIGSRSFQSRLLVGTGKYKDLNETDLAIQASGAEIVTVAIRRVNIGQNPDQPNLLSVIPPEKYTILPNTAGCFDADSAVRTCMLARALLDGHNLVKLEVLGDEKTLYPNVTETLKAARTLIDDGFEIMVYTSDDPIIAQELESMGCVAIMPLGSLIGSGLGILNPHTISII-KENAKVP-VLVDAGVGTASDAAIAMELGCDGVLMNTAIAAAQNPILMASAMKKAVEAGREAFLAGRMPRKRMANASSPETGYFFK 2HTM Chain:A ((6-241)) -------VGPVELKSRLILGSGKYEDFGVMREAIAAAKAEVVTVSVRRVE--------GLLEAL--EGVRLLPNTAGARTAEEAVRLARLGR-LLTGERWVKLEVIPDPTYLLPDPLETLKAAERLIEEDFLVLPYMGPDLVLAKRLAALGTATVMPLAAPIGSGWGVRTRALLELFAREKASLPPVVVDAGLGLPSHAAEVMELGLDAVLVNTAIAEAQDPPAMAEAFRLAVEAGRKAYLAGPMRP----------------

General information: TITO was launched using: RESULT: Template: 2HTM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1309 -128378 -98.07 -565.54 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.82 3D Compatibility (PKB) : -98.07 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.567

(partial model without unconserved sides chains): PDB file : Tito_2HTM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2HTM-query.scw PDB file : Tito_Scwrl_2HTM.pdb: