Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSDSSNQLMPLSLSAPGVNLGAYISTVNQIPILTAEQEKELAER-----------------------------YYYDQDLDAAK-MLVMSHLRFVVHIARSYAGYGLPQGDLIQEGNLGLMKAVKRFDPNMGVRLVSFAVHWIKAEIHEYVIRNWRIVKIAT---------TKAQRKLFFNLRSLKKSSKKLTLEEAQSIANDLNVTPEQVLEMEGRLTAYDAAFEAQGDDDDDTPHTAPVLYLEDNR----YDPARLVENEDWEEQSTSALHDAMNQLDDRSRNILQRRW--LDDDKSTLHELAAEYNVSAERIRQLEKNAMEKIKVAMSAS 5UH5 Chain:F ((221-515)) ------------LTASADSVRAYLKQIGKVALLNAEEEVELAKRIEAGLYATQLMTELSERGEKLPAAQRRDMMWICRDGDRAKNHLLEANLRLVVSLAKRYTGRGMAFLDLIQEGNLGLIRAVEKFDYTKGYKFSTYATWWIRQAITRAMADQARTIRIPVHMVEVINKLGRIQRELLQDL------GREPTPEE---LAKEMDITPEKVLEIQQYAREPISLDQTIGDEGDSQLGD----FIEDSEAVVAVDAVSFTLLQDQ-------LQSVLDTLSEREAGVVRLRFGLTDGQPRTLDEIGQVYGVTRERIRQIESKTMSKLR------

General information: TITO was launched using: RESULT: Template: 5UH5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 655 10008 15.28 40.03 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain F : 0.73 3D Compatibility (PKB) : 15.28 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.430

(partial model without unconserved sides chains): PDB file : Tito_5UH5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5UH5-query.scw PDB file : Tito_Scwrl_5UH5.pdb: