Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEKFVDDHHLKRKVGTLEMVVAMLLSGSIGLFVIKSGQSPINIVFFRCLISALCLIPICYFYGHFRKVYFGKKELFLMVTSGLLIIFNWVLLFAAFPKTSISLATIVYHVNPFVILFLGALVFHEKLNKNDVLWTIIAFIGLIVIIGLGSASVNSNELVGLGLVLIATTLYSISVLITKKLSNTPPLLIVFIQTLSGAIVMAPFISVFEN-PP-IGQQWLFVVGLGVLHTAFLYYLMYSAIKKIPLNNIAILSFIYPISTIVIDYFFFDHVLTSTQVLGAGLILLGVLGVKLHWNIFAVKKVGIRN 5I20 Chain:E ((3-141)) ------------------------------------------------------------------------------------------------------------------------------------------------------------RSSATLIGFTAILLWSTLALATSSTGAVPPFLLTALTFTIGGAVGIAAGLARGVGLSVLRQPWP-VWVHGIGGLFGYHFFYFSALKLAPPAEAGLVAYLWPLLIVLFSAFLPGERLRPAHVAGALMGLAGTVVLLGARAG----------

General information: TITO was launched using: RESULT: Template: 5I20.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 181 -27158 -150.04 -198.23 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain E : 0.51 3D Compatibility (PKB) : -150.04 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.51 QMean score : -0.001

(partial model without unconserved sides chains): PDB file : Tito_5I20.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5I20-query.scw PDB file : Tito_Scwrl_5I20.pdb: