Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQQQVIIVGGGMVGLSLSLMLAKANIAVKLLEAVKYPNYDDQNVAPYHSSFDARNTALSRRSVQIYQKLGLWDALQQHATPILQVHITEQGSFGKARLIAEQEKVESFGQVIENAWLGRVLLTQVRQQPLIELIDGVQVTALTQDAEQVHIEAQRGDEVLKLESKLLIAADGRDSFCRQAIGVG----------------VDVHDYDQVAIVTTVQTSKPHEHVGFERFSALGPLALLPLPGEYRRSVVWPVKKGTEGEWLGEENDQHFLDALQKTYGDRAGK-FEKTGKRF--SYPLSQVLAHKQAVGRIILMGNAAHTIHPVAGQGFNLCLRDADVLLRYLVNQLSASDDIGNPDNLLAYEEARLSDQQRVIKFCDTVVRGFSNQNPLLKLIRNTGLIAFDVIPGVKPLVANYAMGLKA 5KOX Chain:A ((7-326)) ----VIIAGGGPTGLMLAGELRLHGVRTVVLEKEPTPN--------QHS----RSRGLHARSIEVMDQRGLLERFLAHGE---QFRVGGFFAGLAAEWPADLDTAHSYVLAIPQVVTER-LLTEHATELGAEIRRGCEVAGLDQDADGVTAELADG---TRLRARYLVGCDGGRSTVRRLLGVDFPGEPTRVETLLADVRIDVPVETLTAVVAEVRKTQ-------LRFGA------VPAGDGFFRLIV-------PAQGLSADRAAPTLDELKRCLHATAGTDFGVHSPRWLSRFGDATRLAERYRTGRVLLAGDAAHIHPPTGGQGLNLGIQDAFNLGWKLAAAIGG---WAPPDLLDSYHDER-------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5KOX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1634 -120268 -73.60 -399.56 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -73.60 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.418

(partial model without unconserved sides chains): PDB file : Tito_5KOX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5KOX-query.scw PDB file : Tito_Scwrl_5KOX.pdb: