Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLDYLQALKRIPEARGAWILHGQEPLLEQNLLDTFRKSWQQQDIERQRYDISSVSDWKNVFNALNSLSLF-SQQLAIEVHGNIKPDANGLKQLKSYIQHNETNLLLIVLPKQDSSSLKSAFFQVVEANGVVVALTANYPQDRQ--RILAAEAEKLEIQLDNDAWQWLMQHHEHNLLAAKNSLMRVRDTFPDQKLIQIEQLYACLQDQSRYTTYDLSDALLEGNLAQSIKIFQYLIGSGEPESLILWTLSKEMRLLMQLFEQ----PHNAL--QLGIWKTKVSLYQQALRRLNPQQFLGWSALLLQIDAAIKGMSNENAQHLMQQAIAELCGKTLFIH 3GLF Chain:A ((49-331)) ---------------------------------------------EHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTGLLHDDLLLIVRGNKLSKAQENAAWFTALANRSV-QVTCQTPEQAQLPRWVAARAKQLNLELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLT-LPRVEQAVNDAAHFTPFHWVDALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKHRVWQNRRGMMGEALNRLSQTQLRQAVQLLTRTELTLK-------QDYGQSVWAELEGLSLLL-

General information: TITO was launched using: RESULT: Template: 3GLF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 927 -20864 -22.51 -76.14 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -22.51 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.464

(partial model without unconserved sides chains): PDB file : Tito_3GLF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3GLF-query.scw PDB file : Tito_Scwrl_3GLF.pdb: