Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVRINKMNGKHVDIYIGKLNDFLNRKQHDFPDFRTFNEYKKQQIQLIKNKLIAQKLEIAPTDLVFAKHEYGKPYLLDHA-----LHFNHSHSQQYYALALSERVKDIGIDVEELDRKVR-----LDSLAQHAFHPDEYATWQSLEQD---REYWFKVWTTKEAVLKASGLGIRLDLNTLNTQAPPTNHGGLCSHELIGTFAYQNFVLGNMILTVAWRSEQSCRGFQFPSIQIHSLDR 2C43 Chain:A ((74-232)) --------------------------------------------RLMIRKLVAEKLNIPWNHIRLQRTAKGKPVLAKD-NPYPNFNFNISHQGDYAVLAAEPELQ-VGIDIMKTSFPGRGSIPEFFHIMKRKFTNKEWETIRSFKDEWTQLDMFYRNWALKESFIKAIGVGLGFELQRLEFDLSPLN--------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2C43.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 425 5418 12.75 42.32 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : 12.75 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.408

(partial model without unconserved sides chains): PDB file : Tito_2C43.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2C43-query.scw PDB file : Tito_Scwrl_2C43.pdb: