Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDALELKNIISDGLLSFPVTDFDQNGDFNKSSYAKRLEWLAPYGASALFAAGGTGEFFSLTGNEYSEVIKTAVDICKGSVPIIAGAGGPTRQAIEQAKEAERLGAHGILLMPHYLTEASQEGLIEHVKQVCNSVDFGVIFYNRSVSRLNLDSIQKLTEMCPNLIGFKDSSGQIDMMTAVTQTIGDRLSYLGGLPTAEVFAAPYKALGCPVYSSAVFNFIPKTAMEFYNALRSDDFETTNRLIKDFFLPLIKIRDRKSGYAVSMIKAGAKIVGHDAGPVRPPLSDLTQADYEDLAALIATLGPQ 5HWN Chain:A ((1-298)) MDPEQIKTALGSGLLSFPVTHFDAEGRFAADSYREHVEWLAGYKAPVLFAAGGTGEFFSLKPDEIPTIVAAAKEVA-GETAIVSGCGYGTEIAVDIARSVEKVGADGILLLPHYLIDAPQEGLYAHIKKVCQSVGIGVMVYNRDNSVLQADTLARLCDECPNLVGFKDGTGDIGLVRQITAKMGDRLMYLGGMPTAELFAEAYLGAGFTTYSSAVFNFVPGLANEFYAALRAGERATCERILVDFFYPFMAIRNRAKGYAVSAVKAGVRLQGFNAGPVRAPLKDLTNEEIGMLEALIGT----

General information: TITO was launched using: RESULT: Template: 5HWN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1710 -226998 -132.75 -761.74 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.87 3D Compatibility (PKB) : -132.75 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.87 QMean score : 0.602

(partial model without unconserved sides chains): PDB file : Tito_5HWN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5HWN-query.scw PDB file : Tito_Scwrl_5HWN.pdb: