Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLQVKIVPVTAFAQNCSLVWDSETKEAVLID-----AGGDAAVLKKEVEALGLKVKALWLTHGHLDHAGAVGELAKEWSVPVVGPHKEDQFWLDMIQEVSARYGFPIPQPVKVDQWLEGGEVLKLGEDEFEVRFAPGHTPGHVMFYNKNHGLLWTGDVLFKGSIGRTDFPRGNHEQLIESIQRECFSLPDETQFISGHG----PMSTIGYEKQFNPFVAGKAG 4CHL Chain:A ((23-195)) -----------------LLGDRESREAVLIDPVLETAPRDAQLIKE----LGLRLLYAVNTHCHADHITGSGLL---------------RSLLPGCQSVISRL-----SGAQADLHIEDGDSIRFGRFALETRASPGHTPGCVTFVLNDHSMAFTGDALLIRGCGRTDFQQGCAKTLYHSVHEKIFTLPGDCLIYPAHDYHGFTVSTVEEERTLNP-------

General information: TITO was launched using: RESULT: Template: 4CHL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 901 1677 1.86 10.22 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : 1.86 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.622

(partial model without unconserved sides chains): PDB file : Tito_4CHL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4CHL-query.scw PDB file : Tito_Scwrl_4CHL.pdb: