Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIEKIKQAASFLYWGKMMAGFYRTNLGRVALQQRNIALNAKQRHLLLLIDHE-DFQTLNTEFKKR-I--------APPELIQQLIDLKLIAPISENDSEFTEQIPLSESPTTSLEVKAQQKSTIDENESADLTGEIKVSLEPSCHSSNIENTQPPIPVQQLTFEEIQLLMKQSLSQYCGLMAKPLIQKIEQIKNLQELKMCQMQWITSLQESRIPPHELTHTLHSINYSIQLIQQKN 3G2B Chain:A ((37-94)) ---------------------------------RVVELDDIALVVAQRYDGTQSLAQIAQTLAAEFDADASEIETDVIELTTTLHQKRLLR--------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3G2B.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 130 2918 22.44 60.78 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.55 3D Compatibility (PKB) : 22.44 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.55 QMean score : 0.786

(partial model without unconserved sides chains): PDB file : Tito_3G2B.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3G2B-query.scw PDB file : Tito_Scwrl_3G2B.pdb: