Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSFTRSQIFLACALASSLLFSACSKENKPQKEDALTATAPATGEASTIIERNAKQRLIETLQKQFKNANINVKILDIKPTEVPNLYWVNLEGMTSVYTTSDGKYLIQGDVIRLGGKELHNIGDSLQASENKKHLAALKNEDLIAYPAKGGKAKHVIYVFTDVSCPYCHKLHEHLAEINEKSIEVRYIAWPR---GEQFMPTMEAVWCSADRKAAFNQAVQGINIPPAQCKNPVREQYQLGLNMGVNGTPAIYNVDGEYLGGYLTPDELIKRLDK 1JZD Chain:A ((10-208)) -------------------------------------------------------------------AKMGIKSSDIQPAPVAGMKTV-LTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLL----KQLNALEKE-MIVY--KAPQEKHVITVFTDITCGYSHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIWCAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQPPKEMKEFLDE

General information: TITO was launched using: RESULT: Template: 1JZD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 924 -69274 -74.97 -353.44 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -74.97 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.671

(partial model without unconserved sides chains): PDB file : Tito_1JZD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1JZD-query.scw PDB file : Tito_Scwrl_1JZD.pdb: