Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRYILIALLIIVFGYSLALVLQNPTELPVDLLFTQVPAMRLGLLLLLTLALGIVVGLLLGVQVFRVFQKSWEIKRLRKDIDHLRKEQIQSAQLAAAEAAANVRHEKTVVDIYPQDKNSTPL 1SST Chain:A ((123-168)) ---------------SLALYLQNQISVAFDVDIH--PAAKIGHGIMFDHATGIVVG-----------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1SST.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 65 -13731 -211.24 -352.06 target 2D structure prediction score : 0.08 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -211.24 2D Compatibility (Sec. Struct. Predict.) : 0.08 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.166

(partial model without unconserved sides chains): PDB file : Tito_1SST.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1SST-query.scw PDB file : Tito_Scwrl_1SST.pdb: