Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVEKNILNPEAVAEALERAMNLANPQTTHAAIAVPTSTVIHKTIEMDADMSDDEREVQIRVDAEQYIPFPLDEVSLDFEVLP--DRLANPNRVNVLLVATRTENVETRVEVLELADLNPKLADVESYAVERTFSVFADSLPIGANTIGILDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSL---EEASRAKKDRSLPDDYEIEVLD-------PFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFLQMGFSPQ-VDVQKIENDASSLMVACGLALRSFD 2YCH Chain:A ((16-374)) LGLEIGASALKLVEVS--GNPPALKALASRPTPPGLLMEGMVAEPAALAQEIKELLLEARTRKRYVVTALSNLAVILRPIQV-PKMPLKEMEEAVRWEAERYIPFPI-EVVLDFAPLTPLSEVQEGEQVQVMVAAARQEAVAGVLEALRGAGLVPVVLDVKPFAGLYPLEARLAEEP--DRVFLVLDIGAESTSLVLLRGDKPLAVRVLTLSGKDFTEAIARSFNLDLLAAEEVKRTYG--------------RIYDAIRPVLVELTQELRRSLEFFRIQL---SPEVGYLLGGGSKLRGLASLLTDTLGVNLEPVNPWEAVAVDPKRFESEQLQEIGPEFAVALGLALRGVE

General information: TITO was launched using: RESULT: Template: 2YCH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1543 -53713 -34.81 -169.44 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -34.81 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.548

(partial model without unconserved sides chains): PDB file : Tito_2YCH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2YCH-query.scw PDB file : Tito_Scwrl_2YCH.pdb: