Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTIKLNPLNAIDFYKADHRRQYPAGTEYVYANFTPRSSRLAKMLPDFDDKVVFFGLQGFIKHFLIDTWNEGFFKQPKDKVVAAYKRRM-DSSLGEGAVPVDHIEALHDLGYLPLRIKALPEGSRVNMRVPVLTVINTDPRFFWLTNYIETVLSAELWKSCTTATIAYEYKRLLTQYAVKTGAPLDFVPVQGHDFSSRGMSGIYDAAQSGVGHLTSFIGTDSVASIDYAEEYYNATG-VIGVSVPATEHSVMCMGTEDNELETFKRLICELYPSGVVSIVSDTWDFWRVITE-FTVALKPEILARQPNALGLAKLVFRPDSGDPVKIICGDPDAKVGSPAYKGAVECLWEVFGGTTTDQGYKVLNECVGLIYGDSITLDRAQRILEGLETKGFASNNLVFGIGSFTYNYLTRDTFGFAVKATWGQVNGVGRELFKDPITDSGVKKSAKGLLRIEESENG-FTLFDQQTAEQEQGGA--LKTVFENGKLQYECTLDQIRERLSIA 4JNM Chain:A ((8-482)) ---EFNILLATDSYKVTHYKQYPPNTSKVYSYFECREKK-------KYEETVFYGLQYILNKYLK---GKVVTKEKIQEAKDVYKEHFQDDVFNEKGW--NYILEKYD-GHLPIEIKAVPEGFVIPRGNVLFTVENTDPECYWLTNWIETIL-VQSWYPITVATNSREQKKILAKYLLETSGNLDGLEYKLHDFGYRGVSSQETAGIGASAHLVNFKGTDTVAGLALIKKYYGTKDPVPGYSVPAAEHSTITAWGKDHEKDAFEHIVTQ-FSSVPVSVVSDSYDIYNACEKIWGEDLRHLIVSRSTQA----PLIIRPDSGNPLDTVLK-------------VLEILGKKFPVTENSKGYKLLPPYLRVIQGDGVDINTLQEIVEGMKQKMWSIENIAFGSGGGLLQKLTRDLLNCSFKCSYVVTNGLGINVFKDPVADPN-KRSKKGRLSLHRTPAGNFVTLEEGKGDLEEYGQDLLHTVFKNGKVTKSYSFDEIRKNAQL-

General information: TITO was launched using: RESULT: Template: 4JNM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2513 27444 10.92 59.66 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : 10.92 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.473

(partial model without unconserved sides chains): PDB file : Tito_4JNM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4JNM-query.scw PDB file : Tito_Scwrl_4JNM.pdb: