Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLIHLFFRNNKKSKMHLKLNNYLISGEYMNIPRFGLGTFRLKEQAVIDSVKNALDVGYRAIDTAQIYENEAAIGQAIAESGVSRQDLFLTTKIWVDNFAQDKFIPSLKESLQKLRTDHVDLTLIHWPAPDLGVSIPEIMQLLLEAKQQGLTKQIGISNFNIALTQQAIDTIGVEHIATNQIELSPYLQNHKLVNFLQEKNIDVTSYMTLAYGKVLQDPVLAEIAAANKATTAQIALAWALQRGFAVIPSSTKRENLISNLKAQDIELTAAEMQMIAELDRNSREVSPEPWAPVWD 3WBW Chain:A ((25-277)) ------------------------------MPQIGLGVWETPPDETAEVVKEAVKLGYRSVDTARLYKNEEGVGKGLED----HPEIFLTTKLWNDEQGYDSTLRAYEESARLLRRPVLDLYLIHWPMPAQGQYV-ETWKALVELKKSGRVKSIGVSNFESEHLERIMDATGVVPV-VNQIELHPDFQQRALREFHEKHNIRTESWRPLGKGRVLSDERIGKIAEKHSRTPAQVVIRWHLQNGLIVIPKSVNPKRLAENLDVFGFVLDADDMQAIEQMDRKDGRMGADP------

General information: TITO was launched using: RESULT: Template: 3WBW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1369 -102467 -74.85 -405.01 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -74.85 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.566

(partial model without unconserved sides chains): PDB file : Tito_3WBW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3WBW-query.scw PDB file : Tito_Scwrl_3WBW.pdb: