Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTSSNTSRIAVQQIDPHELKAWIKAQALDLGFADCVSAKPDAQEQMPRFLEYLERGYHADMTYLEENLEKRADPTLLVPGTKSIICVRMNYLVESPK-PRYVPFEPNSAITARYARGRDYHKVMRGRLKTLATRIREKVGDFESRPFADSAPIFEKSLAESAGMGWTGKHTLLIHKKSGSLFVLGELFTSLDLPFDEPATSHCGSCSACIDICPTQAIVEPYTLDARRCIAYLTIEYKGIIAEELRAGIGNRIFGCDDCQLICPWNSFAKTASIPDFNPRHGLDNISLLDIWQWDEATFLANTEGSPIRRTGYQSFKRNIAIGLGNAPYSKEIVDQLNKGKSLHDEIVNVHIDWAIEQQLNQL 5D08 Chain:A ((77-291)) --------------------------------------------------------------FEEPDIEKRVTPKLLLPKAKSIVAIALAYPSRMKDAPRSTRTE-RRGIFCRASWGKDYHDVLREKLDLLEDFLKSKHEDIRTKSMVDTGELSDRAVAERAGIGFSAKNCMITTPEYGSYVYLAEMITNIPFEPDVPIEDMCGSCTKCLDACPTGALVNPGQLNAQRCISFLT-QTKGFLPDEFRTKIGNRLYGCDTCQTVCPLNKGKDFHLHPEMEP------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5D08.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 937 -6906 -7.37 -32.27 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -7.37 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.448

(partial model without unconserved sides chains): PDB file : Tito_5D08.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5D08-query.scw PDB file : Tito_Scwrl_5D08.pdb: