Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAFRNNVPQASWRLAKIALGGIVLTGLAVGTYAYAQQKPLPTV-DKVELDRYLGVWYEVARKPAFFQK-KCAYNVSATYTLNENGNIVVDNRCYDNQKQLQQSISEAFVVNPPYNTKLKVSFLPEAVRWIPIIRGDYWILKLDED-----YQTVLVGEPSRKYLWVLSRTPHPHKEVVDEYLNYAKTLGFDIRDIIHTEYKE 5EZ2 Chain:A ((9-158)) --------------------------------------PKPAVQEDFDAARYLGVWYDIQRLPNKFQKGECA---TATYSLSPGVGFSVFNRERLANGTIKSVIGSAIAEDPCEPAKLQ--FFHENAAPVP-----YWVLSTDYDNYALVYSCINLGASHAAYASIVSRQPTLPEETIKKLQGTMSSFGVGVDTLLTT----

General information: TITO was launched using: RESULT: Template: 5EZ2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 646 5199 8.05 36.35 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 8.05 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.403

(partial model without unconserved sides chains): PDB file : Tito_5EZ2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5EZ2-query.scw PDB file : Tito_Scwrl_5EZ2.pdb: