Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQQRVIVGMSGGVDSSVSAALLLQQGYQVEGLFMKNWEEDDGTEYCTAMEDLADAQAVADKIGIKLHTANFAMEYWDRVFEHFLAEYAAGRTPNPDILCNKEIKFRAFLDHAMTLGADFIATGHYARRAETAYNSKGEAYAPLLRGLDNNKDQTYFLHAVHGREINKTLFPVGEIEKPEVRRIAEELDLATAKKKDSTGICFIGERRFNDFLKQYLPAQPGKIVLDNGKEVGEHHGLMYYTLGQRGGIGLGGMKGASEGAWFVLHKDVTNNRLVVGQGHDHPLMQSTQLWSESIDWVAGEQNIPAE-GLRCTAKTRYRQPDQACTVFID-ENSEHGVRVEFDEPQRAVTPGQSVVFYSDEVCLGGGVIHHTNAPTPNFI 2HMA Chain:A ((25-363)) -----------------TALLLKEQGYDVIGIFMKNWDDTD----CTATEDYKDVVAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVMCNKEIKFKAFLDYAITLGADYVATGHYAR---VARDEDGTVHM--LRGVDNGKDQTYFLSQLSQEQLQKTMFPLGHLEKPEVRRLAEEAGLSTAKKKDSTGICFIGEKNFKNFLSNYLPAQPGRMMTVDGRDMGEHAGLMYYTIGQRGGLGIG----GDNAPWFVVGKDLSKNILYVGQGFYHDSLMSTSLEASQVHFT---REMPEEFTLECTAKFRYRQPDSKVTVHVKGEKTE----VIFAEPQRAITPGQAVVFYDGEECLGGGLI-----------

General information: TITO was launched using: RESULT: Template: 2HMA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1611 -117188 -72.74 -356.19 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.84 3D Compatibility (PKB) : -72.74 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.497

(partial model without unconserved sides chains): PDB file : Tito_2HMA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2HMA-query.scw PDB file : Tito_Scwrl_2HMA.pdb: