Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDETKMSYQSIFRPDAFANKVIIVTGGGSGIGRCTAHELAALAAQVVITGRKIEKLEKVSQEII-EDGGLVHFIVCDNREEEQVKNMIAEVIEKFGKLDGLVNNAGGQFPSALENISANGFDAVVRNNLHATFYLMREAYNQWMAKHGGSIVNMTADMWG-GMPGMGHSGAARSGVDNLTKTASVEWGKSGVRVNAVAPGWIV-SSGMDNYSGDFAKVIIPSLAGNVPLKRMGTESEVSSAICYLLSDAAAFVSGVTLRIDGAASQGTRMYPLAEATNSQSYNGFHRAFIPEIFKKDKTEKVEQSNNSGSE 4FC7 Chain:A ((10-270)) -DDCLPAYRHLFCPDLLRDKVAFITGGGSGIGFRIAEIFMRHGCHTVIASRSLPRVLTAARKLAGATGRRCLPLSMDVRAPPAVMAAVDQALKEFGRIDILINCAAGNFLCPAGALSFNAFKTVMDIDTSGTFNVSRVLYEKFFRDHGGVIVNITATLGNRGQALQVHAGSAKAAVDAMTRHLAVEWGPQNIRVNSLAPGPISGTEGLRRLGGPQASLSTKVTAS--PLQRLGNKTEIAHSVLYLASPLASYVTGAVLVADGGA-----------------------------------------------

General information: TITO was launched using: RESULT: Template: 4FC7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1472 -104654 -71.10 -405.64 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -71.10 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.469

(partial model without unconserved sides chains): PDB file : Tito_4FC7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4FC7-query.scw PDB file : Tito_Scwrl_4FC7.pdb: