Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIIGYARTTINDVDLTTQVDQLSDFG--CQEIFHETYEVEQNDSTAVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTRE-MGKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRP-KIDAKTIKKIRRLYYEKKETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI 2GM4 Chain:A ((1-180)) MALFGYARVSTSQQSLDIQVRALKDAGVKANRIFTDKAS----DRKGLDLLRM--KVKEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVSIRFIDDSIDTSSAMGRMVVTILSAVAQAERQRILQRTNEGRQEAMAKGVVFGRKRKIDRDAVLNM----WQQGLGASHISKTMNIARSTVYKVIN-----------

General information: TITO was launched using: RESULT: Template: 2GM4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 554 -13108 -23.66 -76.21 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -23.66 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.435

(partial model without unconserved sides chains): PDB file : Tito_2GM4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2GM4-query.scw PDB file : Tito_Scwrl_2GM4.pdb: