Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPLAIMASEDHSGLIQAVESSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSID--TPFSLFDEANTKDATALSEAMEALSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK 1CUK Chain:A ((87-200)) --LAILSGMSAQQFVNAVEREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTP----------TDDAEQEAVARLVALGYKPQEASRMVSKIARPD-ASSETLIREALR-----

General information: TITO was launched using: RESULT: Template: 1CUK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 320 2936 9.17 29.65 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : 9.17 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.562

(partial model without unconserved sides chains): PDB file : Tito_1CUK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1CUK-query.scw PDB file : Tito_Scwrl_1CUK.pdb: